# Supported reference genome assemblies

**Both GRCh37/hg19 and GRCh38/hg38 are supported.** You can run cases with both reference genomes in the same organization.

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Note: Curated and historical data are automatically lifted over on the fly.
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### For the DRAGEN v4.3 analysis, Emedgene utilizes:

* **GRCh38/hg38**: Multigenome Graph `hg38-alt_masked.cnv.graph.hla.rna-10-r4.0-1.tar.gz`.

  Pre-built multigenome hash tables for hg38. The hash table builds include DNA, RNA, CNV, and HLA tables. Download [here](https://webdata.illumina.com/downloads/software/dragen/resource-files/hg38-alt_masked.cnv.graph.hla.rna-10-r4.0-1.tar.gz).
* **GRCh37d5**: Multigenome Graph `hs37d5-cnv.graph.hla.rna-10-r4.0-1.tar.gz`.

  Pre-built multigenome hash tables for GRCh37d5. The hash table builds include DNA, RNA, CNV, and HLA tables. Download [here](https://webdata.illumina.com/downloads/software/dragen/resource-files/hs37d5-cnv.graph.hla.rna-10-r4.0-1.tar.gz).

### For the DRAGEN v4.2 analysis, Emedgene utilizes:

* **GRCh38/hg38**: Multigenome Graph `hg38-alt_masked.cnv.graph.hla.rna-9-r3.0.tar.gz`.\
  Pre-built multigenome hash tables for hg38. The hash table builds include DNA, RNA, CNV, and HLA tables. Download [here](https://webdata.illumina.com/downloads/software/dragen/references/genome-files/hg38-alt_masked.cnv.graph.hla.rna-9-r3.0-1.tar.gz).
* **GRCh37d5**: Multigenome Graph `hs37d5-cnv.graph.hla.rna-9-r3.0.tar.gz`.

  Pre-built multigenome hash tables for GRCh37d5. The hash table builds include DNA, RNA, CNV, and HLA tables. Download [here](https://webdata.illumina.com/downloads/software/dragen/references/genome-files/hs37d5-cnv.graph.hla.rna-9-r3.0-1.tar.gz).

### For the DRAGEN v4.0 analysis, Emedgene utilizes:

* **GRCh38/hg38**: Multigenome Graph `hg38-alt_masked.cnv.graph.hla.rna-8-r2.0-1.tar.gz`.

  Pre-built multigenome hash tables for hg38. The hash table builds include DNA, RNA, CNV, and HLA tables. Download [here](https://webdata.illumina.com/downloads/software/dragen/hg38%2Balt_masked%2Bcnv%2Bgraph%2Bhla%2Brna-8-r2.0-1.run).
* **GRCh37d5**: Multigenome Graph `hs37d5-cnv.graph.hla.rna.tar.gz`.

  Pre-built multigenome hash tables for GRCh37d5. The hash table builds include DNA, RNA, CNV, and HLA tables. Available on demand.

### For DRAGEN versions < 4.0, Emedgene utilizes:

* **GRCh38/hg38**: `GCA_000001405.15_GRCh38_no_alt_analysis_set.fna.gz`.

  Contains the sequences of the chromosomes, the rCRS mitochondrial sequence, unlocalized scaffolds, and unplaced scaffolds. Download [here](https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/000/001/405/GCA_000001405.15_GRCh38/seqs_for_alignment_pipelines.ucsc_ids/GCA_000001405.15_GRCh38_no_alt_analysis_set.fna.gz).
* **GRCh37/hg19**: `hs37d5.fa.gz`.\
  Includes data from GRCh37, the rCRS mitochondrial sequence, Human herpesvirus 4 type 1 and the concatenated decoy sequences. Download [here](https://ftp.1000genomes.ebi.ac.uk/vol1/ftp/technical/reference/phase2_reference_assembly_sequence/hs37d5.fa.gz).

{% embed url="<https://www.illumina.com/science/genomics-research/articles/dragen-demystifying-reference-genomes.html>" %}


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