Actionability

A single table in the biomarker details provides a list of all the therapeutic, prognostic, and diagnostic assertions from all included sources that have content about the biomarker (for example, My Knowledge Base).

By default, the assertions are filtered by the case and ancestor diseases. Ancestor diseases are determined using SNOMEDCT. These diseases can be viewed at the SNOMED International SNOMED CT Browser website, with the following exceptions:

• Descendents of neoplastic disease do not include ancestors beyond neoplastic disease.

• Non-small cell lung cancer has been added as an ancestor of adenocarcinoma of lung.

You can also filter by other diseases by the summary. When filtering by summary for guideline evidence, specify the website (for example, ESMO.org or NCCN.org).

Actionability Legend

Field Name	Description
Column	Description
Published Date	The date that the assertion was updated.
Source	Knowledge base origin of the assertion.
Biomarker	Indicates whether an assertion is the nucleotide, annotation overlap, partial fusion, amino acid,codon, exon, or gene level. This column is only available for variants.
Classification	This column displays the classification (for example, Tier 1A), as indicated by the knowledge base.
Type	Therapeutic, Diagnostic, or Prognostic as indicated by the knowledge base.
Direction	For therapeutic, responsive, nonresponsive, etc, as indicated by the knowledge base. For prognostic, favorable, or unfavorable, as indicated by the knowledge base.
Therapy	Therapy for the assertion.
Disease	Disease for the assertion.
Actions	This column contains the following available actions: • Add to report • Archive • View past cases To edit an assertion, add it to the report before editing. For more information, refer to Edit,Remove, and Archive Assertions. To remove an assertion from the report, select Archive. If the assertion was added through automation, it is not automatically added again to the same report.
Status	Indicates the TMB, MSI, or GIS status for the assertion. This column is available for TMB, MSI,and GIS.
HRD	Indicates whether an assertion is associated with HRD positive or negative. This column is available for GIS and variants when the selected transcript is for BRCA1/2.

Available Knowledge Bases

Memorial Sloan Kettering OncoKB

Connected Insights provides the Memorial Sloan Kettering OncoKB. For more information, refer to the OncoKB website.

The following from OncoKB are not supported:

• Non-compliant HGVS

• Unspecified positions: Epigenetic Silencing, ARv567es, AR-V7, DNMT3B7, vIII, vII, vV, TGFBR1*6A, Overexpression, Wildtype, Promoter Hypermethylation, Hypermethylation, Kinase Domain Duplication, Internal Tandem Duplication, Partial Tandem Duplication

Jackson Clinical Knowledge Base (JAX-CKB)

Connected Insights provides the Jackson Clinical Knowledge Base (JAX-CKB). For more information, refer to the Jackson CKB website.

The following from JAX-CKB are not supported:

• Complex molecular profiles (for example, co-occuring biomarker assertions)

• Emerging and risk factor evidence types

• Class #, over exp, dec exp, hypermethylation, hypomethylation, dup exonX, dup exonX-X, LOH, loss, negative (except for HRD andMSI), positive (except for HRD), and wild type variants.

Clinical Interpretation of Variants in Cancer (CIViC)

Connected Insights also provides the Clinical Interpretation of Variants in Cancer (CIViC) knowledge base. For more information, refer to the CIViC website.

The following from CIViC are not supported:

• Assertions

• Functional and oncogenic evidence types

• Splice Site (c.3028G>A), Boolean-like evidence (for example, (V600E or V600K) and Amplification), Underexpression, Overexpression, Expression, Decreased Peri-therapeutic Expression, Cytoplasmic Expression, Loss, Biallelic Inactivation, Alu Insertion, Alternative Transcript, RSID, Homozygosity, Copy-neutral Loss of Heterozygosity, Cytoplasmic Mislocation, Deleterious Mutation, Double Ph, Wildtype, Domain, Exon-specific fusions