QA/QC, data processing, and dimension reduction

QA/QC & Data Processing

Once the data has been imported in the project we can start pre-processing the data:

We will first remove all non-expression features in the data (e.g. NegProbes).

  • Click on Filtering > Filter features from the menu on the right

  • Select Metadata and set the task settings as follows

  • Click Finish

In the Analyses tab

  • Click on the resulting filtered counts node

  • Select QA/QC > Single cell QA/QC from toolbox, once the task has completed we can open the report by double-clicking the node:

We will remove the cells with low counts and number of detected features.

  • Click on Select & Filter and set lower threshold to 50 for both (remember that this is data-dependent and will change based on your dataset)

  • Click Filter include

  • Click Apply observation filter to the filtered counts node:

Click on the node generated by the filtering task in the Analyses tab.

  • Click Filtering > Filter features. Apply a noise reduction filter:

We can now normalize our filtered data.

  • Click Normalization and scaling > Normalization. Use the recommended settings by clicking :

Data Exploration

Now that we have filtered low quality cells and normalized our data, we can start clustering to identify cell populations.

  • Click on the normalized data node

  • From the menu on the right select Exploratory analysis > PCA. We are going to use the top 2000 features by variance and calculate the first 50 principal components (PCs):

Once the PCA has run, click on the PCA result node in the Analyses tab.

  • Select Exploratory analysis > UMAP from the toolbox. Set the UMAP parameters as follows:

    • Top 20 PCs

    • Local neighborhood size 60

    • Minimal distance 0.20

While the UMAP is running we can also queue a clustering task. Click on the PCA result node in the Analyses tab, select Exploratory analysis > Graph-based clustering.

  • We are going to use the Leiden algorithm to cluster our data (make sure to select the radio button for it)

  • Set the number of PCs to 10

  • In the advanced settings, set the resolution parameter to 8e-5 and click Apply:

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Additional Assistance

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