Importing Copy Number Data
Last updated
Last updated
This tutorial uses a spreadsheet generated after data import, but we will illustrate the steps used to import the data in this section.
Select Copy Number from the Workflows drop-down menu
Select Import Samples from the Copy Number workflow
The import dialog will open (Figure 1).
Figure 1. Viewing the Import Copy Number Samples dialog
For Affymetrix arrays, Partek Genomics Suite can import CEL files with allele intensity values and calculate copy number estimates from these intensities. For Agilent, Illumina, NimbleGen, or Affymetrix .CHP files, Partek Genomics Suite can import files containing calculated copy numbers or log ratios.
For this tutorial, we will not be importing CEL files.
Select Cancel to close the import dialog
Later sections of this tutorial will address starting with copy number or log ratios and performing GC wave correction on Affymetrix CEL files.
We can now open the tutorial data file.
Download the zipped tutorial data folder Overlapping Copy Number with LOH
Unzip the files to an accessible directory
Select File from the main menu
Select Open...
Select the file IC_Intensities_SNP6.fmt
The spreadsheet will open in the Analysis tab (Figure 2).
Figure 2. Viewing the tutorial data set spreadsheet
This spreadsheet was generated from the import of SNP6 CEL files and shows all 20 samples on rows. Columns 1-6 describe the samples with information such as file names, Subject ID, Gender, etc. The other columns are individual markers from the microarray with the log2 normalized intensities associated with each marker (marker labels are column headers). Opening the IC_Intensities_SNP6.fmt file is equivalent to importing the 20 sample files and adding sample attributes.
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