NextSeq 1000/2000 Integration v2.5.0 supports future ICA services and includes new custom fields and modifications to existing automations. The integration package requires the NextSeq 1000/2000 Sequencing v2.4 workflow. You can install this workflow through the Illumina Preset Protocols (IPP). If you do not want to upgrade through the IPP, you can manually upgrade the workflow configuration.
Modify the Existing NextSeq 1000/2000 Workflow
To modify the existing NextSeq 1000/2000 Sequencing v2.3 workflow, you must make the following changes:
Add the Measurement global field.
Modify the Master Step field.
Modify the Demultiplexing (NextSeq 1000/2000 Sequencing v2.3) master and protocol steps.
Update the Validate Reagent Cartridge Barcode step automation.
Add Index Sequence Global Field
From Configuration, select the Custom Fields tab.
In Global Fields, create a new Measurement field with the following properties:
Field Name — Index Sequencing
Field Type — Text
Required Field — No
Read Only — Yes
Select Save.
Modify Master Step Field
From Configuration, select the Custom Fields tab.
Select the Master Step Fields tab.
Select Demultiplexing (NextSeq 1000/2000 Sequencing v2.3) to display the applicable fields.
Select Criteria 1 - Source Data Field.
In the Additional Options pane, remove # of > = Q30 Bases (PF) from the Dropdown Items list.
Select Criteria 2 - Source Data Field.
In the Additional Options pane, remove # of > = Q30 Bases (PF) from the Dropdown Items list.
When the /opt/gls/clarity/config/configure_nextseq1k2k.sh script is running as part of the installation and configuration process, do not use the default names for the automated sequencing and demultiplexing steps. Instead, use the names in the modified workflow.
To make modifications after configuration, use the omxProps-ConfigTool utility to update the following properties: